Service Analytics 

ESSENCE LINE provides complete bionalytical services essential for monitoring human and animal biomarkers, biomarker discovery and drug development.
In cooperation with academic institutes, we develop original analytical methods and apply them in practise using HPLC-MS/MS, NMR, RT-PCR and microarrays.

Your Benefits

Broad range: Profit from a large assortment of toxicological test allowing a global regulatory submission by the chemical, agrochemical, pharmaceutical and cosmetic industries as well as analysis of environmental samples in the field of biodegradation of ecotoxicology

High speed: Move faster from discovery to market due to immediate analysis of your compound, no waiting list

Efficient: Receive results during all stages of your study

Flexibility: Experience the flexibility by small and smart organizations

Certified: Your studies are performed according to regulatoy guidelines with or without GLP

Experienced: Profit from an extensive and long-term-experience

1. Physico-Chemical properties

UN Methods
• C.1 Metal Corrosivity (Chapter 37.4) 
• H.4 Heat accumulation storage test (Chapter 28.4.4)
• N.1 Test method for readily combustible solids (Chapter 33.2)
• N.2 Test method for pyrophoric solids (Chapter 33.3)
• N.3 Test method for pyrophoric liquids (Chapter 33.3)
• N.4 Test method for self-heating substances (Chapter 33.3)
• N.5 Test method for substances which in contact with water emit flammable gases (Chapter 33.4)
• O.1 Test for oxidizing solids (Chapter 34)
• O.2 Test for oxidizing liquids (Chapter 34)
CIPAC Methods
• CIPAC MT 22 Viscosity
• CIPAC MT 39 Stability of liquid formulations at 0°C
• CIPAC MT 46 Accelerated Storage Procedure
• CIPAC MT 47 Persistent Foaming
• CIPAC MT 53 Wettability
• CIPAC MT 75 pH and pH at 1%
• CIPAC MT 148 Pourability
• CIPAC MT 160 Spontaneity of dispersion
• CIPAC MT 171 Dustiness
• CIPAC MT 172 Flowability of water dispersible granules
• CIPAC MT 174 Dispersibility
• CIPAC MT 176 Dissolution of water soluble bag
• CIPAC MT 178 Attrition resistance of dispersible granules
• CIPAC MT 179 Dissolution degree and solution stability
• CIPAC MT 184 Suspensibility of formulations
• CIPAC MT 185 Wet sieve test
• CIPAC MT 191 Free Acidity and Alkalinity 

2. In Vitro-Toxicology

Skin corrosion 
• OECD 431 In vitro Skin Corrosion (EpiDerm)
• OECD 435 Membrane Barrier Test- Corrosit
Skin irritation
• OECD 439 In vitro Skin Irritation (EpiDerm)
Skin sensitization
• OECD 442C Direct Peptide Reactivity Assay (DPRA)
Eye irritation
• OECD 437 Bovine Corneal Opacity and Permeability-BCOP Test
• In vitro Eye Irritation [HET CAM]
• Human EpiOcularTM Eye Irritation Test (EIT)

Cytotoxicity
• ISO 10993-5 Growth Inhibition Test

3. In Vivo-Toxicology

Acute Toxicity
• B.1 tris / OECD 423 Acute oral Toxicity on rats
• B.3 / OECD 402 Acute dermal Toxicity on rats
• B.2 / OECD 403 Inhalation Toxicity on rats
• B.4 / OECD 404 Skin Irritation on rabbits
• B.5 / OECD 405 Eye Irritation on rabbits
• B.6 / OECD 406 Sensibilisation
• [Magnusson & Kligman / Bühler]
• OECD 429 Local Lymph Node Assay [LLNA]
• OECD 442B Skin Sensitization: Local Lymph Node Assay: BrdU-ELISA
• OECD 442D In Vitro Skin Sensitization (ARE reporter gene assay)
Chronic, Subchronic, Reproduction and Carcinogenicity Toxicity
• B.7 / OECD 407 28 days Toxicity on rats
• B.8 / OECD 408 90 days Toxicity on rats
• B.31 / OECD 414 Prenatal Development Toxicity
• B.35 / OECD 416 2 Generation Study
• OECD 421 Screening for Reproduction Toxicity
• OECD 422 Combined Repeated Dose Toxicity with Reproduction Toxicity
• OECD 443 Extended One-Generation Reproductive Toxicity Study

4. Mutagenicity / Genotoxicity

In vitro - Studies 
• OECD 471 Ames Test /Ames Screening Test (MPF Test; Xenometrix)
• OECD 473 Chromosome Aberration in human lymphocytes
• OECD 487 Micronucleus in human lymphocytes
• OECD 490 Mouse Lymphoma Assay
• OECD 476 HPRT- Hypoxanthine-guanine phosphoribosyl transferase
• ASTM E1687 Modified Ames Test - Standard Test Method for Determining Carcinogenic Potential of Virgin Base Oils in Metalworking Fluids

5. Biodegradability

Ready Biodegradability
• OECD 301A DOC-Die-Away Test
• OECD 301B CO2-Evolution Test (Sturm Test)
• OECD 301C Modified MITI Test (I)
• OECD 301D Closed-Bottle Test
• OECD 301E Modified OECD Screening Test
• OECD 301F Manometric Respirometry Test
• OECD310 CO2-Headspace Test
Inherent Biodegradation
• OECD 302B Zahn-Wellens-Test
• OECD 302C Modified MITI-Tes
Anaerobe Biodegradation
• OECD 311 / EN ISO 11734 Anaerobic degradation
Special biodegradation
• OECD 303A Activated Sludge Units
• DEKRA PSR Plant Growth Test with Cress - Toxicity determination of oil degradation products

6. Environmental Fate

• OECD 303 Simulation Test – Aerobic Sewage Treatment
• OECD 305 Bioaccumulation (Danio rerio) - aquatic study - feed study
• OECD 306 Biodegradability in Seawater
• CEC L-103-12 Degradation of Lubricants with GC-Analysis
14C-radiolabelled and non radiolabelled*
• OECD 307 Aerobic and Anaerobic Transformation in Soil
• OECD 308 Aerobic and Anaerobic Transformation in Aquatic Sediment Systems
• OECD 309 Aerobic Mineralisation in Surface Water – Simulation Biodegradation

7. Ecotoxicology

Aquatic Studies:
Acute Studies
• OECD 201 Toxicity on Alga (72h) (Desmodesmus, Pseudokirchneriella, Cyanobacteria)
• OECD 202 Toxicity on Daphnia (24/48h)
• OECD 203 Toxicity on Fish (96h) (Danio rerio. Onchorhynchus mykiss/Rainbow trout)
• OECD 236 Fish Embryo Acute Toxicity (FET) Test (Danio rerio)
• OECD DRP (2008) Fish Life-Cycle Test (Danio rerio)
Long Term Studies
• OECD 204 Prolonged fish toxicity (14d/28d; Danio rerio)
• OECD 210 Early Life Stage Toxicity (Danio rerio)
• OECD 211 Reproduction-Toxicity (Daphnia magna)
• OECD 214 Short Term Toxicity Test Embryo/Sac-fry
• OECD 215 Juvenile Growth Test (Danio rerio)
• Fish Life-cycle Test (Danio rerio)
Bacteria Inhibition Test
• OECD 209: Anaerobi Activated sludge respiration inhibition testing
• OECD 224: Anaerobic Inhibition of the activity of anaerobic bacteria
• ISO 9509 Nitrification Inhibition Study
Terrestrial Studies
• OECD 207 Earthworm, Acute Toxicity Tests (Eisenia fetida; Eisenia andrei)
• OECD 208 Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test
• OECD 222 Earthworm Reproduction Test (Eisenia fetida; Eisenia andrei)
• OECD 216 Nitrogen Transformation Test
• OECD 217 Carbon Transformation Test
• OECD 218 Sediment-Water Chironomid Toxicity Test using spiked sediment
• OECD 219 Sediment-Water Chironomid Toxicit Test using spiked water
• OECD 225 Sediment Water Lumbriculus Test
• OECD 233 Sediment-Water Chironomid Life-Cycle T oxicity Test Using Spiked Water or Spiked Sediment
• OECD 235 Chironomus sp., Acute Immobilisation Test

8. Analytical Instrumentation

Chemicals
Acc. REACH
• Physico-chemical Properties
• in vitro / in vivo* - Toxicology
• Mutagenicity / Genotoxicity
• Aquatic and Terrestrial Ecotoxicology
• Environmental Fate and Behaviour
• Endocrine Disruptors
Sameness acc. REACH
• GPC *
• UV/VIS
• IR
• 1H-NMR/13C-NMR/solid NMR *
• Mass spectrum:
• GC/MS with Headspace and LC-MS/MS
• GC/HPLC Chromatogram
• ICP-OES
• XRF *
• XRD *
• Titration
• Water content:
• Gravimetric, Karl Fischer, freeze drying*
Analytical chemistry                   
• UV-VIS
• DSC
• FTIR
• Liquid-Scintillation-Counter
• ICP-OES
• TOC
• Titration unit
Chromatography
• GC - FID, ECD, NPD
• GC-MS - EI, Headspace
• HPLC - UV, RI, FLD, ELSD, RAD
• HPLC-MS - ESI, APCI
• LC-MS/MS

9. Endocrine Disruptors

Level 1 Sorting & prioritization based upon existing information
Level 2  In vitro assays providing mechanistic data
Level 3  In vivo assays providing data about single endocrine mechanisms
Level 4  In vivo assays providing data about multiple endocrine mechanisms
Level 5 In vivo assays providing adverse effects data from endocrine & other mechanisms for RA

Level 2 
• Estrogen or androgen receptor binding affinity (YES/YAS Assay)
• Stereoidogenesis in vitro (OECD TG 456)*
• MCF-7 cell proliferation assays (ER ant/agonist)*
• Other assays as appropriate*
Level 3
• Uterotrophic assay (OECD TG 440)*
• Hershberger assay (OECD TG 441)*
Level 4
• Repeated dose studies (OECD407/408)*
• Prenatal developmental toxicity study (OECD 414)*
• Chronic toxicity and carcinogenicity studies (OECD 451-3)*
• Reproductive screening test (OECD 421)*
• Combined 28-day/reproductive screening assay (OECD 422)*
• Fish sexual development test (OECD 234)
• Fish Reproduction Partial Lifecycle Test
• Chironomid Toxicity Test (OECD 218-219)
• Daphnia Reproduction Test (OECD 211)
• Earthworm Reproduction Test (OECD 222)
• Sediment Water Lumbriculus Toxicity Test (OECD 225)
Level 5
• Fish Life Cycle Test (Danio rerio)